2024
Im, C., Kim, M., Kim, J.R., Valgepea, K., Modin, O., Nygård, Y. & Franzén, C.J. Low electric current in a bioelectrochemical system facilitates ethanol production from CO using CO-enriched mixed culture. Frontiers in Microbiology, 15:1438758. doi: https://doi.org/10.3389/fmicb.2024.1438758.
Ingelman, H., Heffernan, J.K., Harris, A., Brown, S.D., Shaikh, K.M., Saqib, A.Y., Pinheiro, M.J., de Lima, L.A., Martinez, K.R., Gonzalez-Garcia, R.A., Hawkins, G., Daleiden, J., Tran, L., Zeleznik, H., Jensen, R.O., Schindel, H., Jänes, J., Reynoso, V., Simpson, S.D., Köpke, M., Marcellin, E. & Valgepea, K. Autotrophic adaptive laboratory evolution of the acetogen Clostridium autoethanogenum delivers the gas-fermenting strain LAbrini with superior growth, products, and robustness. New Biotechnology, 83:1-15. doi: https://doi.org/10.1016/j.nbt.2024.06.002; Preprint.
Heffernan, J., Garcia Gonzalez, R.A., Mahamkali, V., McCubbin, T., Daygon, D., Liu, L., Palfreyman, R., Harris, A., Koepke, M., Valgepea, K., Nielsen, L.K. & Marcellin E. Adaptive laboratory evolution of Clostridium autoethanogenum to metabolize CO2 and H2 enhances growth rates in chemostat and unravels proteome and metabolome alterations. Microbial Biotechnology, 17:e14452. https://doi.org/10.1111/1751-7915.14452
2023
Nwaokorie, U.J., Reinmets, K., de Lima, L.A, Pawar, P.R., Shaikh, K.M., Harris, A., Köpke, M. & Valgepea, K. Deletion of genes linked to the C1-fixing gene cluster affects growth, by-products, and proteome of Clostridium autoethanogenum. Frontiers in Bioengineering and Biotechnology, 11; 1167892; doi: https://doi.org/10.3389/fbioe.2023.1167892; Preprint.
2022
de Lima, L.A., Reinmets, K., Nielsen, L.K., Marcellin, E., Harris, A., Köpke, M. & Valgepea, K. RNA-seq Sample Preparation Kits Strongly Affect Transcriptome Profiles of a Gas-Fermenting Bacterium. Microbiology Spectrum, 31; 10(4):e0230322; doi: https://doi.org/10.1128/spectrum.02303-22; Preprint.
Im, C., Valgepea, K., Modin, O. & Nygård, Y. Clostridium ljungdahlii as a biocatalyst in microbial electrosynthesis – Effect of culture conditions on product formation. Biotechnology Resource Reports, 19, 101156; doi:https://doi.org/10.1016/j.biteb.2022.101156.
de Lima, L.A., Ingelman, H., Brahmbhatt, K., Reinmets, K., Barry, C., Harris, A., Marcellin, E., Köpke, M. & Valgepea, K. Faster Growth Enhances Low Carbon Fuel and Chemical Production Through Gas Fermentation. Frontiers in Bioengineering and Biotechnology, 879578; doi: 10.3389/fbioe.2022.879578; Preprint.
Valgepea, K., Talbo, G., Takemori, N., Takemori, A., Ludwig, C., Mahamkali, V., Mueller, A.P., Tappel, R., Köpke, M., Simpson, S.D., Nielsen, L.K. & Marcellin, E. Absolute Proteome Quantification in the Gas-Fermenting Acetogen Clostridium autoethanogenum. mSystems, doi: 10.1128/msystems.00026-22; Preprint.
Heffernan, J.K., Mahamkali, V., Valgepea, K., Marcellin, E., Nielsen, L.K. Analytical tools for unravelling the metabolism of gas-fermenting Clostridia. Current Opinion in Biotechnology, 28;75:102700; https://doi.org/10.1016/j.copbio.2022.102700
Pavan, M., Reinmets, K., Garg, S., Mueller, A.P., Marcellin, E., Köpke, M., Valgepea, K. Advances in systems metabolic engineering of autotrophic carbon oxide-fixing biocatalysts towards a circular economy. Metabolic Engineering, S1096-7176(22)00021-0; doi: https://doi.org/10.1016/j.ymben.2022.01.015
Doddapaneni, T.R.K.C., Ahmad, F., Valgepea, K., Kikas, T. Integrated thermochemical and biochemical processes for the production of biofuels and biochemicals, In: Biomass, Biofuels, Biochemicals. Elsevier. 67–105. doi: https://doi.org/10.1016/B978-0-323-89855-3.00025-X
2020
Espinosa, M.I., Gonzalez-Garcia, R.A., Valgepea, K., Plan, M.R., Scott, C., Pretorius, I.S., Marcellin, E., Paulsen, I.T. & Williams, T.C. Adaptive laboratory evolution of native methanol assimilation in Saccharomyces cerevisiae. Nature Communications, 11:5564. doi: https://doi.org/10.1038/s41467-020-19390-9; Preprint.
Mahamkali, V.*, Valgepea, K.*, Lemgruber, R.d.S.P., Plan, M., Tappel, R., Köpke, M., Simpson, S.D., Nielsen, L.K. & Marcellin, E. Redox controls metabolic robustness in the gas-fermenting acetogen Clostridium autoethanogenum. Proceedings of the National Academy of Sciences of U.S.A., doi: 10.1073/pnas.1919531117
Heffernan, J.K.*, Valgepea, K.*, Lemgruber, R.d.S.P., Casini, I., Plan, M., Tappel, R., Simpson, S.D., Köpke, M., Nielsen, L.K. & Marcellin, E. Enhancing CO2-valorization using Clostridium autoethanogenum for sustainable fuel and chemicals production. Frontiers in Bioengineering and Biotechnology, 8:204. doi: https://doi.org/10.3389/fbioe.2020.00204; Preprint.
2019
Lemgruber, R.d.S.P., Valgepea, K., Gonzalez Garcia, R.A., de Bakker, C., Palfreyman, R.W., Tappel, R., Köpke, M., Simpson, S.D., Nielsen, L.K. & Marcellin, E. A TetR-Family Protein (CAETHG_0459) Activates Transcription From a New Promoter Motif Associated With Essential Genes for Autotrophic Growth in Acetogens. Frontiers in Microbiology 10:2549; doi: https://doi.org/10.3389/fmicb.2019.02549; Preprint.
Lemgruber, R.d.S.P.*, Valgepea*, K., Tappel, R., Behrendorff, J.B., Palfreyman, R.W., Plan, M., Hodson, M.P., Simpson, S.D., Nielsen, L.K., Köpke, M. & Marcellin, E. Systems-level engineering and characterization of Clostridium autoethanogenum through heterologous production of poly-3-hydroxybutyrate (PHB). Metabolic Engineering, 53: 14-23; doi: https://doi.org/10.1016/j.ymben.2019.01.003
2018
Valgepea, K., Lemgruber, R.d.S.P., Abdalla, T., Binos, S., Takemori, N., Takemori, A., Tanaka, Y., Tappel, R., Köpke, M., Simpson, S.D., Nielsen, L.K. & Marcellin, E. H2 drives metabolic rearrangements in gas-fermenting Clostridium autoethanogenum. Biotechnology for Biofuels, 11:55; doi: https://doi.org/10.1186/s13068-018-1052-9
Lemgruber, R.d.S.P., Valgepea, K., Hodson, M.P., Tappel, R., Köpke, M., Simpson, S.D., Nielsen, L.K. & Marcellin, E. Quantitative analysis of tetrahydrofolate metabolites from Clostridium autoethanogenum. Metabolomics, 14:35; doi: https://doi.org/10.1007/s11306-018-1331-2
2017
Valgepea, K., Lemgruber, R.d.S.P., Meaghan, K., Palfreyman, R.W., Abdalla, T., Heijstra, B., Behrendorff, J.B., Tappel, R., Köpke, M., Simpson, S.D., Nielsen, L.K. & Marcellin, E. Maintenance of ATP homeostasis triggers metabolic shifts in gas-fermenting acetogens. Cell Systems, 4: 505-515; doi: https://doi.org/10.1016/j.cels.2017.04.008
Valgepea, K., Loi, K.Q., Behrendorff, J.B., Lemgruber, R.d.S.P., Plan, M., Hodson, M.P., Köpke, M., Nielsen, L.K. & Marcellin, E. Arginine deiminase pathway provides ATP and boosts growth of the gas-fermenting acetogen Clostridium autoethanogenum. Metabolic Engineering, 41: 202-211; doi: https://doi.org/10.1016/j.ymben.2017.04.007
*joint first author